How I Met Your Monomer: Oligomer Counting for FLImP using RJMCMC

Emily Gribbin, Queen’s University Belfast

Co-authors: Benjamin Davis, Science and Technology Facilities Council; Daniel Rolfe, Science and Technology Facilities Council; Hannah Mitchell, Queen’s University Belfast

Abstract: Determining the distribution of protein oligomers in a sample is crucial to understanding a wide range of biological processes. Fluorescence Localisation Imaging with Photobleaching (FLImP) is a super-resolution imaging technique used for high-precision molecular localisation in protein oligomers, however it does not currently extract oligomer state distributions. FLImP traces are repurposed here for oligomer counting with Reversible Jump MCMC (RJMCMC), providing a richer characterisation of their distributions.